Biofilm formation by Staphylococcus aureus on stainless steel surfaces and evaluation of its sensitivity to sanitizers

This study aimed to assess the ability of adhesion, detachment, and biofilm formation reference strain Staphylococcus aureus CCM 4223 on a stainless steel surface, efficacy three disinfectants: preparation A (based peracetic acid, 20 mg/l), B (newly developed disinfectant with combination two active ingredients, caprylic acid hydrogen peroxide, 25 C sodium hypochlorite, 200 mg/l) in removing S. cells adhered onto this surface. Cell detachment was around 5 log CFU/cm 2 (colony forming units) over first 6 h 48 characterizing high persistence tested The number (5–7 ) needed for noted already after incubation. decrease cell counts caused by 1.12–2.07 ( P < 0.0001), whereas B, 1.13–2.08 1.61–2.16 , respectively 0.0001). Preparation found be most effective (80.9%) reducing count matrix. From these results, assayed revealed capacities adhere form biofilms surfaces, matrixes were resistant total removal when exposed evaluated disinfectants.